Despite appearing confident as he arrived for the first day on Tuesday, body language expert Judi James claims that his attitude appeared visibly different on Wednesday.
After a smooth appearance on day one, the second day was thrown into chaos following an outburst by a supporter - which led to Harry being led out of the courtroom. The woman, who was heard shouting, "I support you Prince Harry", was also led out of the courtroom.
Ms James told the Mirror: "The contrast in Harry's body language signals between day one and day two of his court appearances looked acute.
"Arriving on the first day his nonverbal presentation suggested bravado. His jacket flapped open to show off a puffed or splayed chest and he strode out quickly, throwing a couple of celebrity-style waves to the press."
She added: "On day two though there was an air of more subdued caution plus some suggestion of inner anxiety.
"He held his jacket together with one hand in a barrier or self-comfort ritual and he was seen pausing to glance back as though keen to check his entourage were with him."
Ms James explained further: "Any cockiness from day one seemed to have evaporated and his smile had become a mouth clamp with his brows pulled together in a reflective frown."